pksS [2019-06-25 09:11:16]

cytochrome P450, hydroxylase of the polyketide bacillaene produced by the pks cluster

locus: BSU17230

pI: 6.12

mw: 43.26 kDa

protein length: 405 aa

gene length: 1218 bp

function: polyketide synthesis

product: hydroxylase of the polyketide produced by the pks cluster

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.1|Biosynthesis of antibacterial compounds]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.15|Biosynthesis of antibacterial compounds]

Gene

Coordinates: 1,858,566 → 1,859,783

The protein

Catalyzed reaction/ biological activity

• hydroxylation of dihydrobacillaene [Pubmed|17482575]

Paralogous protein(s)

• [protein|A95C28DEE73AD25E28C45B88A7C03C92835F9705|CypA], [protein|8FBC9A1AC108DC99E275D7A27C18C797D3CDB936|BioI], [protein|D8E72C2523D5E6214C9CBAC2C1B27EA45378E162|YjiB]

Structure

• [PDB|4YZR]

Expression and Regulation

Operons

• genes: [gene|EF0F13BB682791E167EFC350BF029B08E8A1F410|pksS]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE17230 (Δ[gene|EF0F13BB682791E167EFC350BF029B08E8A1F410|pksS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE17230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGCATTCTCCTCGCCT, downstream forward: _UP4_TAACATTCAAAACGCCCCCT
• BKK17230 (Δ[gene|EF0F13BB682791E167EFC350BF029B08E8A1F410|pksS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK17230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTGCATTCTCCTCGCCT, downstream forward: _UP4_TAACATTCAAAACGCCCCCT

References

• 17482575,21821766